array-based analysis of dna methylation patterns Search Results


humanmethylation27 bead array  (INFINIUM Inc)
90
INFINIUM Inc humanmethylation27 bead array
DNA-methylation profiles were analyzed with the <t>HumanMethylation27</t> BeadChip microarray which represents 27,578 unique CpG sites. MSC derived from adipose tissue (MSC-AT) were compared with those derived from bone marrow, which was either aspirated from the iliac crest (MSC-BM) or taken from the caput femuris upon hip replacement (MSC-Hip). Unsupervised principal component analysis (PCA) clearly separated DNA-methylation profiles according to the tissue of origin in the first dimension (PC1), whereas the forth component (PC4) discerned early and late passage ( A ). Scatterplot comparison of passage 5 and passage 10 in MSC-AT revealed that 233 CpG sites are more than 15% hyper-methylated whereas 186 CpG sites are more than 15% hypo-methylated at passage 10 ( B ). Significance Analysis of Microarray (SAM) was used to select 517 senescence-associated CpG sites (FDR = 4.8%) and these are presented as a heatmap ( C ; data were divided by the mean of each row for graphical presentation).
Humanmethylation27 Bead Array, supplied by INFINIUM Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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illumina infinium methylationepic bead chip array  (DIAGENODE DIAGNOSTICS)
90
DIAGENODE DIAGNOSTICS illumina infinium methylationepic bead chip array
DNA-methylation profiles were analyzed with the <t>HumanMethylation27</t> BeadChip microarray which represents 27,578 unique CpG sites. MSC derived from adipose tissue (MSC-AT) were compared with those derived from bone marrow, which was either aspirated from the iliac crest (MSC-BM) or taken from the caput femuris upon hip replacement (MSC-Hip). Unsupervised principal component analysis (PCA) clearly separated DNA-methylation profiles according to the tissue of origin in the first dimension (PC1), whereas the forth component (PC4) discerned early and late passage ( A ). Scatterplot comparison of passage 5 and passage 10 in MSC-AT revealed that 233 CpG sites are more than 15% hyper-methylated whereas 186 CpG sites are more than 15% hypo-methylated at passage 10 ( B ). Significance Analysis of Microarray (SAM) was used to select 517 senescence-associated CpG sites (FDR = 4.8%) and these are presented as a heatmap ( C ; data were divided by the mean of each row for graphical presentation).
Illumina Infinium Methylationepic Bead Chip Array, supplied by DIAGENODE DIAGNOSTICS, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/illumina infinium methylationepic bead chip array/product/DIAGENODE DIAGNOSTICS
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custom affymetrix based dna microarray  (Thermo Fisher)
99
Thermo Fisher custom affymetrix based dna microarray
FIGURE 1. Purification of B cells and T cells from mixed splenocytes before and after activation. CD8 T cells, CD4 T cells, and B cells were purified from mixed splenocytes before or after activation using the MACS system with anti-CD8-, anti-CD4-, or anti-CD19-coated magnetic beads, respectively. Phenotypic analysis of purified fresh and activated cell populations was performed by flow cytometry and showed 90% purity of all cell preparations, as indicated. Purified cells were used either for RNA preparation for <t>microarray</t> analysis, or were processed to extract glycans for MALDI profiling and methylation analysis.
Custom Affymetrix Based Dna Microarray, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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crispr based dcas9 suntag dnmt3a system  (Addgene inc)
95
Addgene inc crispr based dcas9 suntag dnmt3a system
FIGURE 1. Purification of B cells and T cells from mixed splenocytes before and after activation. CD8 T cells, CD4 T cells, and B cells were purified from mixed splenocytes before or after activation using the MACS system with anti-CD8-, anti-CD4-, or anti-CD19-coated magnetic beads, respectively. Phenotypic analysis of purified fresh and activated cell populations was performed by flow cytometry and showed 90% purity of all cell preparations, as indicated. Purified cells were used either for RNA preparation for <t>microarray</t> analysis, or were processed to extract glycans for MALDI profiling and methylation analysis.
Crispr Based Dcas9 Suntag Dnmt3a System, supplied by Addgene inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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crispr based dcas9 suntag dnmt3a system - by Bioz Stars, 2026-05
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microarray dna methylation signature  (Epimmune Inc)
90
Epimmune Inc microarray dna methylation signature
FIGURE 1. Purification of B cells and T cells from mixed splenocytes before and after activation. CD8 T cells, CD4 T cells, and B cells were purified from mixed splenocytes before or after activation using the MACS system with anti-CD8-, anti-CD4-, or anti-CD19-coated magnetic beads, respectively. Phenotypic analysis of purified fresh and activated cell populations was performed by flow cytometry and showed 90% purity of all cell preparations, as indicated. Purified cells were used either for RNA preparation for <t>microarray</t> analysis, or were processed to extract glycans for MALDI profiling and methylation analysis.
Microarray Dna Methylation Signature, supplied by Epimmune Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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allprep dna rna mini kit  (Qiagen)
99
Qiagen allprep dna rna mini kit
FIGURE 1. Purification of B cells and T cells from mixed splenocytes before and after activation. CD8 T cells, CD4 T cells, and B cells were purified from mixed splenocytes before or after activation using the MACS system with anti-CD8-, anti-CD4-, or anti-CD19-coated magnetic beads, respectively. Phenotypic analysis of purified fresh and activated cell populations was performed by flow cytometry and showed 90% purity of all cell preparations, as indicated. Purified cells were used either for RNA preparation for <t>microarray</t> analysis, or were processed to extract glycans for MALDI profiling and methylation analysis.
Allprep Dna Rna Mini Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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allprep dna rna mini kit - by Bioz Stars, 2026-05
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27k bead array  (INFINIUM Inc)
90
INFINIUM Inc 27k bead array
FIGURE 1. Purification of B cells and T cells from mixed splenocytes before and after activation. CD8 T cells, CD4 T cells, and B cells were purified from mixed splenocytes before or after activation using the MACS system with anti-CD8-, anti-CD4-, or anti-CD19-coated magnetic beads, respectively. Phenotypic analysis of purified fresh and activated cell populations was performed by flow cytometry and showed 90% purity of all cell preparations, as indicated. Purified cells were used either for RNA preparation for <t>microarray</t> analysis, or were processed to extract glycans for MALDI profiling and methylation analysis.
27k Bead Array, supplied by INFINIUM Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/27k bead array/product/INFINIUM Inc
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dna methylation array-based platform  (GoldenGate Software Inc)
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GoldenGate Software Inc dna methylation array-based platform
FIGURE 1. Purification of B cells and T cells from mixed splenocytes before and after activation. CD8 T cells, CD4 T cells, and B cells were purified from mixed splenocytes before or after activation using the MACS system with anti-CD8-, anti-CD4-, or anti-CD19-coated magnetic beads, respectively. Phenotypic analysis of purified fresh and activated cell populations was performed by flow cytometry and showed 90% purity of all cell preparations, as indicated. Purified cells were used either for RNA preparation for <t>microarray</t> analysis, or were processed to extract glycans for MALDI profiling and methylation analysis.
Dna Methylation Array Based Platform, supplied by GoldenGate Software Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/dna methylation array-based platform/product/GoldenGate Software Inc
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array-based dna methylation analysis  (INFINIUM Inc)
90
INFINIUM Inc array-based dna methylation analysis
FIGURE 1. Purification of B cells and T cells from mixed splenocytes before and after activation. CD8 T cells, CD4 T cells, and B cells were purified from mixed splenocytes before or after activation using the MACS system with anti-CD8-, anti-CD4-, or anti-CD19-coated magnetic beads, respectively. Phenotypic analysis of purified fresh and activated cell populations was performed by flow cytometry and showed 90% purity of all cell preparations, as indicated. Purified cells were used either for RNA preparation for <t>microarray</t> analysis, or were processed to extract glycans for MALDI profiling and methylation analysis.
Array Based Dna Methylation Analysis, supplied by INFINIUM Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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array-based dna methylation analysis - by Bioz Stars, 2026-05
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genomestudio software  (INFINIUM Inc)
90
INFINIUM Inc genomestudio software
FIGURE 1. Purification of B cells and T cells from mixed splenocytes before and after activation. CD8 T cells, CD4 T cells, and B cells were purified from mixed splenocytes before or after activation using the MACS system with anti-CD8-, anti-CD4-, or anti-CD19-coated magnetic beads, respectively. Phenotypic analysis of purified fresh and activated cell populations was performed by flow cytometry and showed 90% purity of all cell preparations, as indicated. Purified cells were used either for RNA preparation for <t>microarray</t> analysis, or were processed to extract glycans for MALDI profiling and methylation analysis.
Genomestudio Software, supplied by INFINIUM Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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epic dna methylation beadchip arrays  (INFINIUM Inc)
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INFINIUM Inc epic dna methylation beadchip arrays
FIGURE 1. Purification of B cells and T cells from mixed splenocytes before and after activation. CD8 T cells, CD4 T cells, and B cells were purified from mixed splenocytes before or after activation using the MACS system with anti-CD8-, anti-CD4-, or anti-CD19-coated magnetic beads, respectively. Phenotypic analysis of purified fresh and activated cell populations was performed by flow cytometry and showed 90% purity of all cell preparations, as indicated. Purified cells were used either for RNA preparation for <t>microarray</t> analysis, or were processed to extract glycans for MALDI profiling and methylation analysis.
Epic Dna Methylation Beadchip Arrays, supplied by INFINIUM Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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array-based infinium beadchip  (INFINIUM Inc)
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INFINIUM Inc array-based infinium beadchip
FIGURE 1. Purification of B cells and T cells from mixed splenocytes before and after activation. CD8 T cells, CD4 T cells, and B cells were purified from mixed splenocytes before or after activation using the MACS system with anti-CD8-, anti-CD4-, or anti-CD19-coated magnetic beads, respectively. Phenotypic analysis of purified fresh and activated cell populations was performed by flow cytometry and showed 90% purity of all cell preparations, as indicated. Purified cells were used either for RNA preparation for <t>microarray</t> analysis, or were processed to extract glycans for MALDI profiling and methylation analysis.
Array Based Infinium Beadchip, supplied by INFINIUM Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


DNA-methylation profiles were analyzed with the HumanMethylation27 BeadChip microarray which represents 27,578 unique CpG sites. MSC derived from adipose tissue (MSC-AT) were compared with those derived from bone marrow, which was either aspirated from the iliac crest (MSC-BM) or taken from the caput femuris upon hip replacement (MSC-Hip). Unsupervised principal component analysis (PCA) clearly separated DNA-methylation profiles according to the tissue of origin in the first dimension (PC1), whereas the forth component (PC4) discerned early and late passage ( A ). Scatterplot comparison of passage 5 and passage 10 in MSC-AT revealed that 233 CpG sites are more than 15% hyper-methylated whereas 186 CpG sites are more than 15% hypo-methylated at passage 10 ( B ). Significance Analysis of Microarray (SAM) was used to select 517 senescence-associated CpG sites (FDR = 4.8%) and these are presented as a heatmap ( C ; data were divided by the mean of each row for graphical presentation).

Journal: Aging (Albany NY)

Article Title: Replicative senescence of mesenchymal stem cells causes DNA-methylation changes which correlate with repressive histone marks

doi:

Figure Lengend Snippet: DNA-methylation profiles were analyzed with the HumanMethylation27 BeadChip microarray which represents 27,578 unique CpG sites. MSC derived from adipose tissue (MSC-AT) were compared with those derived from bone marrow, which was either aspirated from the iliac crest (MSC-BM) or taken from the caput femuris upon hip replacement (MSC-Hip). Unsupervised principal component analysis (PCA) clearly separated DNA-methylation profiles according to the tissue of origin in the first dimension (PC1), whereas the forth component (PC4) discerned early and late passage ( A ). Scatterplot comparison of passage 5 and passage 10 in MSC-AT revealed that 233 CpG sites are more than 15% hyper-methylated whereas 186 CpG sites are more than 15% hypo-methylated at passage 10 ( B ). Significance Analysis of Microarray (SAM) was used to select 517 senescence-associated CpG sites (FDR = 4.8%) and these are presented as a heatmap ( C ; data were divided by the mean of each row for graphical presentation).

Article Snippet: Subsequently, we have compared DNA-methylation profiles with the Infinium HumanMethylation27 Bead Array and the profiles differed markedly in MSC derived from adipose tissue and bone marrow.

Techniques: DNA Methylation Assay, Microarray, Derivative Assay, Methylation

FIGURE 1. Purification of B cells and T cells from mixed splenocytes before and after activation. CD8 T cells, CD4 T cells, and B cells were purified from mixed splenocytes before or after activation using the MACS system with anti-CD8-, anti-CD4-, or anti-CD19-coated magnetic beads, respectively. Phenotypic analysis of purified fresh and activated cell populations was performed by flow cytometry and showed 90% purity of all cell preparations, as indicated. Purified cells were used either for RNA preparation for microarray analysis, or were processed to extract glycans for MALDI profiling and methylation analysis.

Journal: Journal of immunology (Baltimore, Md. : 1950)

Article Title: Activation of murine CD4+ and CD8+ T lymphocytes leads to dramatic remodeling of N-linked glycans.

doi: 10.4049/jimmunol.177.4.2431

Figure Lengend Snippet: FIGURE 1. Purification of B cells and T cells from mixed splenocytes before and after activation. CD8 T cells, CD4 T cells, and B cells were purified from mixed splenocytes before or after activation using the MACS system with anti-CD8-, anti-CD4-, or anti-CD19-coated magnetic beads, respectively. Phenotypic analysis of purified fresh and activated cell populations was performed by flow cytometry and showed 90% purity of all cell preparations, as indicated. Purified cells were used either for RNA preparation for microarray analysis, or were processed to extract glycans for MALDI profiling and methylation analysis.

Article Snippet: 6 The online version of this article contains supplemental material. at B row n U niversity on M ay 30, 2014 http://w w w .jim m unol.org/ D ow nloaded from Microarray analysis of the expression of cytokine and glycan transferase genes For gene expression analysis, mRNAs were extracted from purified cell populations of fresh and activated lymphocytes and subjected to analysis on a custom Affymetrix-based DNA microarray containing murine and human glycosyltransferase, sulfotransferase, and cytokine genes, made available by the Consortium for Functional Glycomics.

Techniques: Activation Assay, Magnetic Beads, Cytometry, Microarray, Methylation